At a meeting last week, Ultima Genomics said it could provide human genomes for $100 a pop—one-fifth the current going rate.
. But for years, most sequencing has relied on machines from a single company, Illumina.
Ultima streamlined the process by spraying the DNA-laden beads by the billions onto round silicon wafers the size of dessert plates. Nozzles above each wafer gently squirt out bases and other reagents, which spread thinly and evenly across the wafer as it rotates, reducing the amount of these expensive materials needed. Instead of moving back and forth across under the camera, the disk moves in a spiral, akin to how a compact disk is played, which speeds up imaging.
Until now, cost has limited such single-cell studies, causing a bottleneck in research. But Snyder found Ultima’s low-cost approach enabled him to sequence multiple colon cancer cells to document how one DNA modification, methylation, changes as colon cancer develops. But Lior Pachter, a computational biologist at the California Institute of Technology, has reservations about the new technology. He and graduate student A. Sina Booeshaghi looked at one of the most active genes in blood cells from Levin’s team, a possible cancer biomarker also known for producing a protein athletes sometimes inject to illegally enhance their performance. The Ultima technology sometimes missed the active gene, Pachter says.
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