Chem-map profiles drug binding to chromatin in cells
) were determined by the first derivative maxima of relative fluorescence unit value against time, and ∆was calculated by baseline correction of melting temperatures subtracting control group. A one-site binding model in GraphPad Prism 8 was used to fit FRETU2OS cells were plated in a 12-well plate in DMEM medium supplemented with 10% FBS. After 18 h incubation, cells were treated for 6 h with doxorubicin , Dox-btn1 , Dox-btn2 and 0.1% DMSO , respectively, in fresh medium.
Beads-bound cells were gently washed twice with wash buffer before resuspending in 100 µl probe-1st Ab precomplex solution and incubating at 4 °C overnight at 600 rpm.pA–Tn5 assembled with DNA adapters were prepared as described in ref.. For 5 samples, 2.5 μl 2nd Ab and 5 μl pA–Tn5 transposome were added to 200 μl Dig-300 buffer and incubated on ice for 1 h . A total of 300 μl antibody buffer was added to the 2nd–Tn5 complex solution.
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