Control of acute myeloid leukemia by a trifunctional NKp46-CD16a-NK cell engager targeting CD123
viable cells, was analyzed by flow cytometry and the absolute concentrations of cytokines released into the supernatant were analyzed by mesoscale discovery assay.
Antitumor activity against human MOLM-13 AML cells injected into severe combined immunodeficiency mice For NK cell depletion, 100 µl of polyclonal anti-asialo-GM1 antibody was injected intraperioneally into recipient mice at the indicated time points.A qualified flow cytometry panel composed of antibodies against the antigens CD45 , CD14 , CD203c , CD193 , IgE , CD123 , CD33 and the viability marker Zombie Nir was used to evaluate the phenotype and counts of basophils and total CD123-positive immune cells in cynomolgus monkey blood and bone marrow samples.
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