Discovering molecular 'team-work' underlying nitrate assimilation in a unicellular red alga tokodai_news frontiersin
The same holds true for Cyanidioschyzon merolae, a unicellular red alga, which serves as an excellent model of photosynthetic higher organisms to study transcription. While it is known that the TF called 'CmMYB1' is responsible for transcribing
Moreover, they found that ND controls the subcellular localization and promotor binding capacity of CmMYB1 under +N condition. Chromatin immunoprecipitation analyses confirmed the role of ND in reducing the binding capacity of CmMYB1 to promoter regions of nitrate assimilating genes under +N condition.
Through several analyses using a CmNDB1 knockout strain, they discovered that CmNDB1 deletion resulted in nuclear localization of CmMYB1 and reduced promoter binding capacity of CmMYB1 under +N condition. Moreover, they discovered that CmNDB1 deletion increases the transcription of nitrate assimilation genes under +N condition.
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