DisP-seq reveals the genome-wide functional organization of DNA-associated disordered proteins
DNase in the culture medium for 30 min at 37 °C, then washed with PBS twice. Cell pellets were resuspended in 50 μl freezing media and transferred in an isopropyl alcohol chamber at −80 °C overnight. The next day, the frozen cell pellets were thawed and first incubated in L1 buffer ) for 3 min then resuspended in L2 buffer ), centrifugated and resuspended in tagmentation buffer , 2.5 μl Tn5 transposase , 16.5 μl PBS, 0.5 μl 1% digitonin, 0.
For protein purification, pellets were resuspended in 20 ml lysis buffer with 1 mg mllysozyme rotated at 4 °C for 30 min, and sonicated by QSONICA Q700 sonicator at 4 °C. After centrifugation at 18,400for 10 min at 4 °C, the supernatant cell lysates were filtered through a 0.45 μm filter and then loaded onto a Chromatography Column with 2 ml Ni Sepharose , which was pre-equilibrated in wash buffer at 4 °C.
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