Bloodvessels originating from lymphaticvessels are found to be tailored to specific functions nature
vasculature is present in stage IV AFs. p,p′, Intravascularly injected Qdot705 are detected in all vessels of the adult AF. Scale bars, 20 μm , 30 μm , 50 μm , 100 μm , 200 μm , 500 μm . TD, Thoracic duct, CCL, Collateral cardinal lymphatic, PCV, Posterior cardinal vein.in ECs. Cre recombinase allows expression of different fluorescent proteins in non-recombinant animals. b, Schematic representation of thelineage tracing protocol used in this study.
h-h″, Confocal images ofshowing distinct labelling of lymphatic components in the trunk starting from 24 h after Cre induction. h, ECs in the early TD of 3.5 dpf embryo labelled in green , are detected 24 h after heat and 4-OHT treatments. h′-h″, Reiterative imaging of the same embryo at 4.5 dpf and 5.5 dpf shows the growing TD composed of green ECs . i-j′, Assessment of the stability of the fluorophore ratios after Cre induction.
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