Nature research paper: Histone H2B.8 compacts flowering plant sperm through chromatin phase separation
infection for transient expression. Two days after injection, the leaf epidermis was isolated and stained by DAPI solution containing 0.2% Triton X-100. Images were obtained using a Leica SP8X confocal microscope.seedlings were collected and incubated for 3 h in 50% PEG6000 1× PBS for drought treatment or 1× PBS for controls.Leaf tissue was ground in liquid nitrogen and suspended in 4 ml histone extraction buffer ).
Nuclei were separated from debris by filtering through two layers of miracloth . For pollen nuclei, we collected approximately 20 ml of open flowers and isolated pollen in Galbraith buffer . Nuclei were released by vertexing pollen with glass beads in 200 µl of nuclei isolation buffer at 2,000 r.p.m. for 3 min. The homogenate was filtered through 40-µm and 10-µm cell strainers successively to obtain nuclei.
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