The human brain, with 86 billion neurons, is a remarkable computational marvel. Enter LIONESS, a new tool that reveals its architecture like never before.
Using minimal sample information, the first deep learning step fills in additional information on the brain tissue's structure in a process called Image Restoration. This approach achieves a gentle real-time imaging resolution of around 130 nanometers.
The tool successfully integrates functional measurements with structural observations by imaging calcium ion fluxes and measuring cellular electrical activity. It also benefited from the information provided by human cerebral organoids— miniature brain models that mimic human brain development. Another known technique is light microscopy, which can observe intact tissue without sectioning but lacks resolution for cellular details.
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