The research group of Professor Yoav Shechtman from the Israel Institute of Technology Faculty of Biomedical Engineering has developed groundbreaking technology enabling scientists to see dynamic processes in living cells. Their study was published in Nature Methods.
enabled researchers to observe sub-cellular structures such as organelles, but at the cost of a long acquisition time—a minute or more per image—so whatever was being looked at needed to be held perfectly still.
How does one find what they are looking for under a microscope? In biology, scientists commonly use fluorescent dyes to stain specific structures of interest. This creates high-contrast images of the labeled structures, which can then be seen clearly. There is, however, a physical limit on how good a resolution one can achieve using this methodology. It cannot resolve objects smaller than 200nm—approximately half the wavelength of visible light.
And just as a photo—or even more so, a video—of a child in play or an athlete mid-jump is truer to life than the postured old photographs, scientists need to see the cell and the organelles within it move, respond to stimuli, and do the things that they naturally do., but they move with a certain regularity," Prof. Shechtman explained."If we look at microtubules, for example, they are sort of like threads, bound together into a mesh.
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