The Carrick Glen clinic in Ayr, which was bought for £1.8m, will specialise in orthopaedics.
Mr Yousaf, who announced the purchase during a visit to University Hospital Ayr, said the NTCs in Highland, Forth Valley and Fife will begin operating the next 12 months.
Dr Joellene Mitchell, consultant anaesthetist and co-clinical lead for the NTC Ayrshire & Arran, said she was excited about Carrick Glen becoming part of the NHS.
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