Researchers have pioneered a 3D-SPI method that allows high-resolution imaging of microscopic objects, presenting a transformative approach for future biomedical research and optical sensing. A research team led by Prof. Lei Gong from the University of Science and Technology (USTC) of the Chinese
Scientists have developed a groundbreaking three-dimensional single-pixel imaging technique based on 3D light-field illumination. This method enables high-resolution imaging of microscopic objects. The 3D-SPI approach can potentially revolutionize the visualization of various biological absorption contrasts, cell morphology, and growth, presenting new opportunities in biomedical research and optical sensing.
A research team led by Prof. Lei Gong from the University of Science and Technology of the Chinese Academy of Sciences and collaborators developed a three-dimensional single-pixel imaging approach based on 3D light-field illumination, which enables volumetric imaging of microscopic objects with a near-diffraction-limit 3D optical resolution. They further demonstrated its capability of 3D visualization of label-free optical absorption contrast by imaging single algal cells in vivo.
To exceed the resolution limitation, the researchers built a 3D-LFI-SPM prototype. As a result, the prototype achieves an imaging volume of ~390×390×3,800 μmand a resolution of up to 2.7 μm laterally and 37 μm axially. They performed label-free 3D imaging of livingPotential Applications
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