A new method developed to reread a single polypeptide many times overcomes one of the biggest obstacles toward single-molecule proteomics. Read more in a new SciencePerspective:
Most sequencing methods for nucleic acids require multiple copies of the target molecules. The copying of nucleic acids, through polymerase chain reaction , enabled next-generation sequencing technology that uses more finite targets. This has allowed the development of genomics and transcriptomics approaches to profile single cells.
Proteins are arguably more important for the proper function of living systems, and so proteomics methods that provide the identity, quantity, and sequence of proteins in a single cell is a key goal. However, one obstacle in protein sequencing is our inability to make identical copies of proteins. On page 1509 of this issue, Brinkerhoff
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