Developing cells likely can 'change their mind' about their destiny UniOfBath NatureComms
Real time quantitative PCR was performed with TaqMan™ Fast Advanced Master Mix, according to the manufacturer’s protocol using StepOnePlus™ Real-Time PCR Systems . Ct values were estimated using an automatic baseline and a standard threshold of 0.2. Forrpl13Pigment cell enrichmentwith minor modifications. Briefly, fish were anesthetized with Tricaine, decapitated and digested using TrypLE Express 10 ml per 100 embryos.
cDNA was synthesized using 1 μl of total RNA from melanocytes or iridophores with iScript™ Advanced cDNA Synthesis Kit following by 6 cycles of pre-amplification with pooled 47 pairs of MTE primers designed for NanoString CodeSet ; amplified dsDNA was analysed using the NanoString expression protocol.
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